Assisted large fragment insertion by Red/ET-recombination (ALFIRE)—an alternative and enhanced method for large fragment recombineering
نویسندگان
چکیده
منابع مشابه
Assisted large fragment insertion by Red/ET-recombination (ALFIRE)—an alternative and enhanced method for large fragment recombineering
Functional genomics require manipulation and modification of large fragments of the genome. Such manipulation has only recently become more efficient due to the discovery of different techniques based on homologous recombination. However, certain limitations of these strategies still exist since insertion of homology arms (HAs) is often based on amplification of DNA sequences with PCR. Large qu...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2007
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkm250